目的：建立虚汗停颗粒的HPLC色谱指纹图谱。方法：采用DiamonsilC18 (250 × 4.6 mm, 5 μm)色谱柱，以乙腈-0.1%磷酸为流动相，梯度洗脱，柱温为28℃，检测波长为260 nm，流速为1.0 mL/min，并进行相似度评价、聚类分析和主成分分析。结果：本色谱条件下虚汗停颗粒各成分得到较好的分离，12批有效期内的虚汗停颗粒HPLC指纹图谱有16个共有峰，2批过期虚汗停颗粒有22个共有峰，12批有效期内的虚汗停颗粒与2批过期虚汗停颗粒间有15个共有峰，各批次的相似度大于0.89，聚类和主成分分析可将过期样品单独归为1类。结论：HPLC指纹图谱具有方法简单、重复性好、特征性强等特点，可用于虚汗停颗粒的质量评价。
Objective: To establish a HPLC fingerprint of Xuhanting granules. Method: The analysis was performed on DiamonsilC18 column (250 × 4.6 mm, 5 μm), with acetonitrile-0.1% phosphoric acid solution as the mobile phase, gradient elution. The flowrate was 1 mL/min, column temperature was 28？C and the detection wavelength was set at 206 nm. The similarity evaluation, cluster analysis and principal component analysis were performed on the experimental data. Result: The components of Xuhanting granules were separated well under the condition of this chromatography. There were 16 peaks in the HPLC fingerprint of Xuhanting granules in 12 batches of validity period, 22 peaks in 2 batches of expired Xuhanting granules, and 15 common peaks in 12 batches of validity period and 2 batches of expired Xuhanting granules. The similarity of each batch was more than 0.89. The cluster and principal component analysis classified the expired samples into 1 category. Conclusion: The HPLC fingerprint method established in this paper has the advantages of simplicity, good repeatability and strong characteristics, which can be used for the quality evaluation of Xuhanting granules.
