荷叶生物碱对脂肪变性HepG2细胞脂质积聚的影响
Effect of Alkaloid Extract from Lotus Leaf on Lipid Accumulation in HepG2 Cell

DOI: 10.12677/HJFNS.2021.102010, PP. 72-79

Keywords: 荷叶生物碱，脂质积聚，HepG2细胞，非酒精性脂肪肝
Lotus Leaf Alkaloid, Lipid Accumulation, HepG2 Cell, Nonalcoholic Fatty Liver Disease

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Abstract:

本文以油酸诱导HepG2细胞脂肪变性构建非酒精脂肪肝(Nonalcoholic fatty liver disease, NAFLD)体外细胞模型，通过测定细胞存活率、胞内TG含量、胞内脂滴油红O染色情况和脂质代谢相关基因mRNA表达研究荷叶生物碱提取物(Lotus leaf extract, LLE)对NAFLD脂质积聚的影响。结果显示，LLE对脂肪变性HepG2细胞的IC₅₀为73.77 μg/mL，在无细胞毒性的浓度下，LLE可明显降低脂肪变性HepG2细胞中甘油三酯(Triglyceride, TG)含量和脂滴积聚程度，且高浓度作用更为明显。进一步研究发现，LLE对脂肪变性HepG2细胞中SREBP-1c和ACCα的mRNA表达影响不大，但能显著上调脂肪变性HepG2中PPARα、CPT-1和ACOX1的mRNA表达水平(p < 0.05)。综上，LLE可抑制脂肪变性HepG2细胞增殖，降低细胞中TG含量和脂滴积聚，且该作用与TG的β-氧化分解有关。
In order to study the effect of lotus leaf extract (LLE) on liver fat accumulation induced by Nonalcoholic fatty liver disease, oleic acid-induced HepG2 cells was established and cell viability, TG contents and staining of lipid droplet and the expression of lipid metabolism related genes mRNA were detected. The results showed that the IC₅₀ of LLE on oleic acid-induced HepG2 cells were 73.77 μg/mL. LLE significantly reduced the content of TG and the lipid droplets in oleic acid-induced HepG2 cells. LLE had little effect on the mRNA expression of SREBP-1c and ACC, while it significantly increased the mRNA expression of PPAR α, CPT-1 and ACOX1 in oleic acid-induced HepG2. It is concluded that LLE is effective on inhibiting on cell viability and to reduce lipid accumulation in oleic acid-induced HepG2 cells, which is more related to the up-regulation of lipid β-oxidative.

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