斑点追踪技术评价健心颗粒对心衰大鼠左室功能的影响
Two-Dimensional Speckle Tracking in Evaluation of Effect of Jianxin Granule on Left Ventricle Cardiac Function in Rats with Heart Failure

目的：探讨斑点追踪技术评价健心颗粒对心衰大鼠左室功能的影响。方法：42只SD大鼠，随机分为假手术组、模型组、健心颗粒组和苯那普利组进行干预。假手术组游离腹主动脉后不结扎，生理盐水灌胃。模型组和药物干预组缩窄腹主动脉法制备心力衰竭大鼠模型。对造模成功大鼠于给药前及给药12周行常规超声心动图检查；采用斑点追踪技术分析左室心肌收缩期整体纵向应变(global longitudinal strain, GLS)和周向应变(global circumferential strain, GCS)峰值。酶联免疫吸附试验法测定血浆血管紧张素II (AngII)、N末端前脑钠肽(NT-proBNP)水平；原位缺口末端标记检查左室肌细胞凋亡；MASSON染色分析左室肌纤维化。结果：与假手术组比较，模型组左室射血分数降低，左室扩大，GLS和GCS应变值增高，血AngII、NT-proBNP增高，差异具有统计学意义(p < 0.05)。健心颗粒或苯那普利治疗后，上述指标均改善。结论：斑点追踪技术能定量评价心衰大鼠左室功能，为健心颗粒治疗心力衰竭提供了新的理论依据。
Objective: T0 observe the effect of Jianxin Granule on left ventricle cardiac function in rats with heart failure by using two-dimensional speckle tracking (STI). Methods: Forty-two SD rats were randomly divided into sham operation group, model group, Jianxin Granule group, benazepril group. In the sham operation group, the abdominal aorta was free without ligation, and normal saline was given by gavage. Heart failure was induced by abdominal aorta constriction in model group and drug intervention groups. On the 8th day after operation, the rats were administered with normal saline, Jianxin granules, benazepril. After 12 weeks, left ventricular function was detected by ultrasound; the longitudinal strain and circumferential strain of left ventricular was monitored with STI. The levels of angiotensin II (AngII) and N-terminal pro brain natriuretic peptide (NT-proBNP) were measured by enzyme-linked immunosorbent assay (ELISA) in plasma; the apoptosis of left ventricle myocytes and fibrosis was analyzed by TdT-mediated dUTP nickend labeling and Masson staining respectively. Results: Compared with the sham operation group, the model group showed reduced left ventricular ejection fraction (LVEF), enlarged left ventricle, increased GLS and GCS, higher AngII and NT-ProBNP in plasma. The difference was statistically significant (p < 0.05). After treatment with Jianxin Granule or benazepril, all the above indicators were improved. Conclusion: STI technology can quantitatively evaluate left ventricle function of rats; Jianxin Granule can improve left ventricle function of rats with heart failure and provides a new theoretical basis for Jianxin Granule in treating heart failure.