油茶叶提取物对金黄地鼠皮脂分泌的影响及其作用机制
Effect of Camellia oleifera Leaves Extract on the Development of Sebum Secretion in Golden Hamsters and Its Mechanism

DOI: 10.12677/HJFNS.2021.102015, PP. 114-124

Keywords: 油茶叶提取物，皮脂腺，雄性激素，5α-还原酶，固醇调节元件结合蛋白
Camellia oleifera Leaves Extract, Sebaceous Gland, Androgen, 5α-Reductase, Sterol Regulatory Element Binding Protein

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Abstract:

目的：探讨油茶叶提取物(Camellia oleifera Leaves Extract, COLE)对金黄地鼠皮脂分泌的影响及其作用机制。方法：以金黄地鼠皮脂腺斑为研究载体，设置COLE低、高剂量组，空白对照组，丹参酮胶囊阳性对照组，进行为期28天灌胃实验。观察不同时间段COLE对金黄地鼠皮脂腺斑外观、显微结构、皮脂分泌的影响，测定比较各组血清、皮脂腺斑组织中脂质含量和雄性激素含量，最后通过研究SRD5A1/AR/ SREBP-1c信号通路探究COLE物抑制皮脂腺以及皮脂分泌的作用机制。结果：与空白对照组比较，COLE能够减小皮脂腺面积使其颜色变浅，显著缩小皮脂斑面积(p < 0.05)。HE染色结果显示COLE能够抑制皮脂腺的生长。油红O染色以及游离脂肪酸、甘油三酯、总胆固醇结果显示COLE显著能降低油脂的分泌水平。雄激素测定结果显示COLE能极显著降低金黄地鼠血清以及皮脂腺斑组织中双氢睾酮含量。Western blot实验结果证明COLE能够降低SRD5A1、AR、SREBP-1c蛋白表达的水平。结论：COLE通过抑制SRD5A1/AR/SREBP1c信号通路降低金黄地鼠皮脂腺脂质分泌，限制皮脂腺的发育进而改善皮脂腺斑的性状。
Objective: To investigate the effect of Camellia oleifera Leaves Extract (COLE) on the sebum secretion in golden hamsters and its mechanism. Method: Using golden hamster sebaceous gland spots as the research carrier, the low and high dose groups of COLE, the blank control group, and the tanshinone capsule positive control group were set up for a 28-day gavage experiment. Observe the effects of COLE on the appearance, microstructure, and sebum secretion of the sebaceous plaques in golden hamsters at different time periods, then measure and compare the lipid content and androgen content in the serum, sebaceous plaque tissues of each group, and finally through the study of SRD5A1/AR/SREBP-1c signaling pathway explores the mechanism of COLE substances inhibiting sebaceous glands and sebum secretion. Results: Compared with the blank control group, COLE can reduce the area of sebaceous glands and make the color lighter. HE staining results show that COLE can inhibit the growth of sebaceous glands. Oil red O staining and the results of free fatty acids, triglycerides, and total cholesterol show that COLE can reduce the level of oil secretion. Androgen results show that COLE can significantly reduce the content of dihydrotestosterone in golden hamster serum and sebaceous plaque tissue. The results of Western blot experiments proved that COLE can reduce the expression levels of SRD5A1, AR, and SREBP-1c proteins. Conclusion: COLE inhibits the SRD5A1/AR/SREBP1c signaling pathway to reduce lipid secretion in the sebaceous glands of golden hamsters, restrict the development of sebaceous glands and improve the traits of sebaceous gland plaques.

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