Determining Genetic Variation of Calpastatin Gene with MspI and NcoI Enzymes by Using PCR-RFLP Method in Kivircik Lambs

The aim of this study was to determine the genetic variation of calpastatin gene by using PCR-RFLP method with MspI and NcoI enzymes in Kivircik lambs. Blood samples of Kivircik lambs (n=153) that were collected from eight different farms located in Kirklareli province, were used for DNA isolation. After PCR amplification, products were digested with MspI and NcoI enzymes to differentiate M and N alleles on 2% and 3% agarose gel electrophoresis. The frequency of M and N alleles were found 88.2% and 11.8% for MspI locus and 98.7% and 1.3% for NcoI locus respectively. The frequencies of MM, MN and NN genotypes were identified 0.77, 0.22 and 0.01 respectively for MspI locus. The frequencies of MM and MN genotypes were determined 0.97 and 0.03 respectively for NcoI locus, NN genotype was not observed. Observed heterozygosity was higher in MspI (0.22) than NcoI locus (0.03). MspI and NcoI loci were found in Hardy-Weinberg equilibrium in Kivircik lambs raised in Kirklareli province