Investigations on Grapevine Fanleaf Disease and Relationships Grapevine Fanleaf Virus (GFLV) With Vector Nematode Xiphinema spp. at Vineyards in the Trakya Region of Turkey

Grapevine fanleaf disease caused by Xiphinemanematode transmitted Grapevine fanleaf virus (GFLV) has been the most important disease on grapes (Vitis viniferaL.) in the vineyards of the World. In order to determine Grapevine fanleaf disease caused by GFLV on grapevine stocks and the relationships between virus and vector nematodes Xiphinema spp,this research study was performed during the years of 2013 and 2014. This study was implemented by examining infected stocks and collecting samples in villages and districts of Tekirdag, K？rklareli, and Edirne provinces in the Trakya Region. During the survey visits grapevine leaves, shoots, pediols and cambium tissue samples were collected from virus infected stocks. Beside tissue samples, soil samples with hairy roots were collected from rhizosfer around each infected vine grape stocks. For the identification of GFLV Double Antibody Sandwich Enzyme-Linked Immunosorbent Assay (DAS-ELISA) test was implemented to tissue samples as morphological and morphometrical measurements were applied to soil samples for the identifications of nematode species. As a result of DAS-ELISA tests 35 out of 152 tissue samples revealed the presence of GFLV. Nine soil samples of 35 GFLV infected stocks revealed the presence of X. indexvector. Thirteen soil samples from GFLV infected stocks, however had X. pachtaicumas 13 soil samples were lack of any Xiphinema species at all. Those GFLV infected stocks without nematode vector probably got virus during thegrafting and pruning or at the vineyard establishment with infected saplings. Another results of this study also implied that X. pachtaicumhas been more widespread nematode vector species than X. indexfor GFLV in the Trakya Region