C-Reactive Protein and Serum Amyloid A in Male Dogs after Orchiectomy

The acute-phase response is considered part of the innate immune system. During acute phase response, concentrations of acute phase proteins occur. Therefore, acute-phase proteins are part of the innate immune response and its biological function, although variable, generally relate to defense to pathological damage and restoration of homeostasis. Their levels fluctuate in response to inflammation and tissue injury. The main acute-phase proteins in dogs are C-reactive protein (CRP) and serum amyloid A (SAA). Surgical trauma has effect on many parts of immunological and hematological profile. The aim of this study was to determine the perioperative dynamics of CRP and SAA in male dogs undergoing elective orchiectomy. Blood samples were collectedby jugular venipuncture in the following order: before the surgery (day zero), on first (day one), third (day three) and seventh (day seven) postoperative day (POD). As markers of systemic inflammation, CRP and SAA levels was determined by ELISA using commercial kits. The study showed that the CRP and SAA changes rapidly. The peak CRP and SAA concentrations were detected on the first day after surgery. Serum CRP concentration on the seventh postoperative day was within physiological ranges, while SAA concentration was significantly higher. In conclusion, SAA and CRP measurements above basal levels are clearly indicative of systemic inflammation in dogs