Evaluation of Patients with Anti-HIV ？ Reactivity with Confirmation Test Results

The algorithm used widely in the diagnosis of HIV infection in our country is after screening the samples by micro-ELISA, sending them to reference laboratory for confirmation. The aim of this study was to evaluate the demographic data and the ELISA/confirmation test results of newly diagnosed HIV patients and to contribute to the data in our country. Between January 2014 and December 2018 Anti-HIV ？ Micro ELISA test were studied in the Microbiology Laboratory of Sakarya University Training and Research Hospital. Reactive samples were sent to the reference laboratory for confirmation. The results were scanned retrospectively and the data were recorded including the age and gender of the patients, and the clinic where they were sent. Of 254 patients with anti-HIV ？ reactivity; 192 (75.5%) were male and 62 (24.4%) were female. The most reactivity detected age group was 26-35. Of the 173 confirmed results, 155 (89.6%) were male and 18 (10.4%) were female. Of the 78 patients with incompatible results, 42 (53.8%) were female. All of the reactive results confirmed as positive were found to be greater than 5. The highest false reactivity value was 13.3; the lowest true reactivity value was 5.29. The gender that HIV positivity was more frequent was male and the age group was 26-35. False positivity was higher in females. It can be explained by the conditions causing false positivity are more frequently associated with women. Test results were found incompatible in 78 samples. If the cut-off value was determined to be 5.0 instead of 1.0, no positive patient would be overlooked, but the sample sent unnecessarily to confirmation would be 14 instead of 78. However, under this value there may be true positivities (such as the initial stages of disease) and it’s a screening test so its current cut-off is still in use