Evaluation of the effect of different desensitizers on dentin tubules: An in vitro scanning electron microscopy analysis

Aim: The aim of this in vitro study is to evaluate the persistence of the combined and single applications of cyanoacrylate (Periacryl) and tetra-calcium phosphate + dicalcium phosphate containing desensitizer (Teethmate) and their effect on dentin tubules. Material and Methods: 140 dentin samples were obtained from impacted third molars. The samples were divided into 7 groups (n = 20). Samples without any application named as K (control), samples were subjected to 6% citric acid solution for 1 min; PS (Periacryl) TS (Teethmate) PTS (combined application). Samples were immersed in artificial saliva for 24 hours named as; TY (Teethmate) PY (Periacryl) PTY (combined application). When the application was completed, the preparations were evaluated by scanning electron microscopy in terms of morphological structures, open dentin tubule areas and open dentin tubule diameters. Results: The area of open dentin tubules decreased in PS, PY, PTS and PTY groups compared to the control group (p> 0.05). The mean diameter of the open dentin tubule in the samples was significantly decreased in the PS, PY, PTY, PTS and TY groups compared to the control group (p> 0.05). While there was no statistically significant difference in citric acid treated groups, the mean dentin tubule diameter was significantly lower in the group with artificial saliva than the TY group in the PTY group (p> 0.05). Conclusion: Periacryl is a preparation that can be used in the treatment of dentin sensitivity, however, it increases the retention of Teethmate in combined use