Oxidative Damage of Chronic Water Avoidance and the Effect of Fulvic Acid in Male Rat Tissues with Erectile Dysfunction

Objectives : We aimed to utilize the antioxidant property of fulvic acid in our study to prevent penile tissue damage associated with erectile dysfunction. Material and Methods: 18 Sprague-Dawley male adult rats were distributed into 3 experimental groups: Control (C), Chronic Stress (CS) and Water Avoidance Stress+Fulvic Acid (CS+FA). The histological sections obtained from the testes has been stained with the Hematoxylin&Eosin (H&E) and Masson Trichrome and endothelial and n nitric oxide sentase (eNOS &nNOS) immünohistochemistry labelling has been made to. Total antioksidant status (TAS), total oksidant status (TOS), oxidative stress (OSI), catalase (CAT), glutation peroksidase (GPx) and superoxide dismutase (SOD) levels has been evaluated biochemically. Results: In CS group were examined, it was observed that trabecular endothelial cells are lost, collagen fibers had shrunk, connective tissue and smooth muscle distributions were unevenly distributed compared to the control group. When CS + FA group were examined, damage in the CS group showed considerable recovery in tissues that were treated. In CS group both eNOS and nNOS reactivity had decreased compare to the control group whereas caveolin-1 reactivity had increased. histochemical data. It was also observed increased TOS and OSI levels and decreased TAS, SOD, CAT and GPx levels in the KS group. Conclusion: The fulvic acid used was found to have therapeutic effects but the dosage used for treatment was not sufficient. We suggest to work at different but higher dosages to ensure that the therapeutic effect is achieved