Pathologic Examination of the Protective Effect of Glycyrrhizin on Cyclophosphamide-induced Hemorrhagic Cystitis in Rats

Cyclophosphamide (CP) is a widely used anti-neoplastic agent in humans and small animals and also has some adverse effects in them. The most common adverse effect of CP is hemorrhagic cystitis (HS). The use of Mesna may prevent HS, but is insufficient for some side effects. In this study, the effect of glycyrrhizin (GLY), which is one of the active substances of licorice root, was examined against CP-related side effects. The experiment was designed with 49 male Sprague Dawley rats. The Control group received only physiological saline and the CP group only CP. CP+Mesna group received CP and also three dose of Mesna. The animals in the CP+GLY100 and CP+GLY200 groups were received three doses of GLY, 100 mg/kg and 200 mg/kg dose, respectively. The previous protocol was applied to the CP+Mesna+GLY100 and CP+Mesna+GLY200 groups and only the first GLY application was replaced with Mesna. In this study, it was recorded that bladder macroscopy and histopathology were better preserved in GLY applicated groups compared to CP group. However, it was observed that this protection was slightly weaker than the Mesna and GLY-Mesna combinations. From this point of view, it was observed that GLY could be preventive in CP-related HS, in addition it could also support to Mesna applications