Investigation of MBL-1 gene polymorphism in some cattle breeds raised in Turkey

The concern in using genetic methods in forming resistant herds against diseases in farm animals has recently been increasing. Mannose-binding lectin-1 (MBL-1) gene is one of the genes searched in this kind of research because of this gene's important responsibilities in the immune system. In the presented research, Zavot (n= 81, Z), Anatolian Black (n= 87, AB), Eastern Anatolian Red (n= 72, EAR), Turkish Gray (n= 54, TG), Southern Anatolian Red (n= 44, SAR) which are the cattle breeds raised in Turkey, and Black Switzerland (n= 61, BS) and Simmental (n= 65, S) which are Europe originated cattle breeds were used as the research material. In the research, it was aimed at genotyping the samples of cattle searched in terms of total three single nucleotide polymorphism in the intron 1 (1252 G>A) and exon 2 (2534 G>A, 2569 T>C) regions of MBL-1 gene. In the end of the study, it was found that Hardy Weinberg (HWE) equilibrium broke down in EAR and S breeds (p<0.05) in terms of 1252 G>A coded SNP; in NB and BS races (p<0.001 and p<0.01 respectively) in terms of 2534G>A coded SNP. It was determined that all breeds are in HWE in terms of 2569 T>C coded SNP. This research is the first research where SNPs in intron 1 (1252 G>A) and exon 2 (2534 G>A, 2569 T>C) regions of MBL-1 gene in the cattle breeds raised in Turkey have been searched. At the end of the study, it was thought that the studies where the relationships between SNPs and important raising disease are searched should be planned