Genetic diversity in some Lysiphlebus confusus (Hymenoptera: Braconidae: Aphidinae) populations

Lysiphlebus confusus Tremblay & Eady (Hymenoptera: Braconidae: Aphidiinae) is an important aphid parasitoid that has a wide host range. Genetic diversity in L. confusus was studied by the PCR-RFLP method in terms of host aphid, host plant, and altitude in Adana and Hatay Provinces, Turkey. Restriction sites of SacI, AccI, HpyCH4III, and EcoP151 enzymes on the 709 bp mitochondrial COI gene region was detected by PCR-RFLP method. The sequences were compared with specimens from other countries (Serbia, China, France and Iran) and their net divergence were determined. While the Balcal？ (11) and Ceyhan (4) populations were close to the Iran specimen, Erzin (5) was close to the Serbian and other Iran specimens. The specimens of Cucumis melo L. from Seyhan, Solanum nigrum L. from Gülek (Pozant？), Citrullus lanatus (Tunb.) from Saimbeyli, S. nigrum from Ceyhan, and Vicia sativa L. from Balcal？ were digested with SacI, HpyCH4III, EcoP151, and both EcoP151 and AccI by PCR-RFLP, respectively. Specific genetic diversity related to altitude, host aphid and plant species was not determined