Transferability of Barley Retrotransposons (Sukkula and Nikita) to Investigate Genetic Structure of Pimpinella anisum L.

Transferability of barley retrotransposons (Nikita and Sukkula) were examined in Pimpinella anisum L. seeds by using a retrotransposon-based molecular marker: IRAP (inter-retrotransposon amplified polymorphism). Furthermore, transposons’ sequences identified in medically important plants were obtained form NCBI, and multiple alignment analyses were performed to investigate the evolutionary relationships. These two retrotransposons were identified in Pimpinella anisum L., showing homomorphic band profiles. In addition, limited similar sequences were detected as a result of clustal analyses. Till date, no study about retrotransposons evaluation using IRAP as molecular marker has been published in aniseed. Our results are expected to contribute a new perspective for genome architect of medically important plants in addition to aniseed