The Effect of Different Combinations of Benzyl Amino Purine and Gibberellic Acid on Micropropagation of Rotala rotundifolia (Buch-Ham. ex Roxb) Koehne

Rotala rotundifolia (Buch-Ham. ex Roxb) Koehne is an important aquatic medicinal plant for the treatment of diseases in the traditional medicine system. In this study, in vitro shoot regeneration from the nodal explants of R. rotundifolia in culture medium containing different combinations of 6-benzyl amino purine (BAP) and gibberellic acid (GA3) was investigated. First shoot formation was observed after 10 days in culture medium. At the end of eight weeks, the trial was completed and regeneration data were obtained. Shoot regeneration rate is listed as 72.22-100.00%. 100% shoot regeneration rate was recorded in MS medium including 0.25-0.75 mg/L BAP + 0.25 mg/L GA3. The maximum shoots per explant (18.38) and the longest shoots (2.36 cm) were obtained in cultures with 0.25 mg/L BAP + 0.25 mg/L GA3. No rooting study was carried out due to the rooting of the shoots in the multiplication environment. The acclimation of regenerated shoots to the aquarium environment has been successfully achieved. This report is important in that it offers multiple production of medicinal plant R. rotundifolia. In the future, it can help with the production of secondary metabolites and gene transfer studies