Effect of Some Cytokinins on the Culture Initiation of Cherry Buds

In this study, the effect of different concentrations benzylaminopurine (BAP), thidiazuron (TDZ) and kinetin on culture initiation from cherry lateral buds was investigated. Buds was taken from one-year branches during the dormant period of 5-year-old trees belonging to 0900-Ziraat variety. Concentrations of 0.1, 0.5, 1.0, 2.0 and 4.0 mgl-1 of each of the three cytokinins were used and in addition to these concentrations, 0.05 mgl-1 was used for TDZ. It has been understood that the addition of kinetin to culture medium up to a concentration of 4 mgl-1 does not have a positive effect in order to initiate culture from cherry buds. The most successful results in the types and concentrations of cytokinins used were taken from 2 mgl-1 BAP and 2 mgl-1 TDZ supplemented media. However, the viability of rosette plants grown in TDZ containing media has decreased towards the end of the culture period. In the BAP supplemented medium, more vigorous and healthy rosette shoots were obtained. In cultures containing 2 mgl-1 BAP in the medium, the rate of subculture explants was 80.0 ± 9.2%, the rosette shoot rate was 43.8 ± 12.8% and the number of leaves was 1.71 ± 0.36