Nitrate and Nitrite Levels in Serum and Seminal Plasma in Horses

Nowadays, the fertility of the growing horses is of great importance. The aim of our study is to determine and compare the level of nitric oxide oxidation products of these animals in serum and seminal plasma. Seven healthy British horses were used in the study. Blood samples were taken from the vena jugularis. The sera were removed by centrifugation. Seminal plasmas of ejaculates taken with artificial vagina were separated. Seminal plasma and serum were obtained and nitrate nitrite values were determined. Nitrate and nitrite levels of samples were measured by coupling reagent reagent method. Nitrate levels in both semis and seminal plasma were 6,34 ± 2,7 and 4,95 ± 1,4, respectively. Nitrite levels were higher than seminal plasma in seminal plasma (p≤ 0.05), while nitrite levels in seminal plasma were higher than serum (p≤ 0.05). As a result, seminal plasma and serum nitrate-nitrite values in British stallions were used. Presented to the knowledge of clinical veterinary medicine