Expression patterns of ROS responsive genes on B-stressed canola (Brassica napus ssp. oleifera L.) following selenium treatment

Though mitigating effect of selenium (Se) on various abiotic stresses is apparent, the knowledge on B-stress is very scant. Therefore, this work attempted to reveal its effects on B-stressed canola (Brassica napus ssp. oleifera L.) plants. In the present study, foliar gene expression and amounts of major antioxidant enzymes were investigated under different concentrations of individual (B or Se) and combined (B + Se) applications. The individual applications (per L) included 1.5 g B, 3 g B, 3 mg Se and 6 mg Se, while the combined applications were 1.5 g B+3 mg Se, 1.5 g B+6 mg Se, 3 g B+3 mg Se and 3 g B+6 mg Se. Under the given treatments, APX, CAT, SOD, POD and GR genes were mostly downregulated while some also showed upregulation such as APX (1.5 g B+6 mg Se and 3 g B+6 mg Se), SOD (1.5 g B+3 mg Se), CAT (3 g B, 3 mg Se and 3 g B+6 mg Se) and POD (1.5 g B). Assaying also implicated that beneficial effect of Se might be associated with the presence of stressor; otherwise, Se itself might induce the antioxidant mechanism as stressor. This work provides the results of individual and combined effects of B and Se applications on antioxidant gene expressions and protein activities in canola