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ISSN: 2333-9721
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-  2019 

Biodiversity of Penicillium species isolated from Edirne S??ütlük Forest soil (Turkey)

Keywords: Penicillium,ITS,Toprak,Fungus,Sanger dizileme,Edirne

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Abstract:

We studied Penicillium species isolated from forest soil and aimed to be detected with morphologically, colonial-molecular methods and species diversity. Soil sample was taken Edirne S??ütlük Forest in October, 2015 and 10 cm deep under aseptic conditions and Waksman’s ‘Soil Dilution Method’ was used for the isolation of Penicillium species; some books and articles have been used for morphological and colonial identification. The service was purchased for the molecular diagnosis of fungi and the company used the Bio-Speedy ? Fungal DNA Isolation Kit for isolation. As PCR Kit, Bio-Speedy?, yeast and mould real-time PCR rapid detection kit was used. The obtained sequences were subjected to BLAST analysis similar sequences using the gene bank and to draw phylogenetic tree, used Mega 7 software. Twelve species belonging to the genus Penicillium were identified; these are: P. chrysogenum, P. citrinum, P. commune, P. manginii (current name: Penicillium atrosanguineum), P. ubiquetum (New record for Turkey), P. camemberti, P. sanguifluum, P. janczewskii, P. canescens, P. roseopurpureum, P. steckii (current name: Penicillium citrinum), P. hordei

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