Determination of Total Antioxidant Capacities by Ferrous Thiocyanate Method in Leaf And Seed of Cherry Laurel (Prunus laurocerasus L)

Prunus laurocerasus, also known as taflan or karayemi？, is commonly found in the Black Sea region. This study was carried out to determine total antioxidant capacity of P. laurocerasus plant collected leaves and fruits from Ordu province in August 2016. The collected samples were separated into leaves and fruit kernel, and dissolved in methanol and ethanol solvents to obtain separate extracts. The total antioxidant capacity was measured spectrophotometrically at 12 h in extracts prepared according to the ferrous thiocyanate method. The highest percent inhibition occurred at 84 hours for methanol extracts. The percent inhibition value for the methanol extract of P. Laurocerasus was determined to be 66.97 and 67.10 for the leave and kernel, respectively. The ethanol extracts of leaves and seeds showed the highest percent inhibition at 48th hour, reaching 94.67 and 91.06. Percent inhibition values of the butylated hydroxy toluen (BHT) and trolox were calculated as 39.85 and 43.81 in 48th hour. It was determined that the leaf and kernel of P. laurocerasus showed high antioxidant activity compared to standard antioxidants. It was also found that the ethanol extract gave higher antioxidant activity than the methanol extract. The kernel of the P. Laurocerasus has higher antioxidant capacity than the leaf