Micropropagation of bible hyssop (Origanum syriacum L. var. bevanii (Holmes) Ietswaart)

The aim of this research was to determine the regeneration protocol for micropropagation of Origanum syriacum L. var. bevanii using tissue culture (in vitro) method. In the research, the various explants (leaf disc, stem node, apical and axillary buds) from the donor plants were cultured on Murashige and Skoog (MS) basal medium supplemented with alone and combinations of different concentrations of 2,4-Dichlorophenoxy Acetic Acid (2,4-D) or Naphthalene Acetic Acid (NAA) (0, 0.25, 0.5, 0.75, 1.0 mg/l) and 6-Benzil Amino purine (BAP) or Furfuryladenine (Kinetin) (0, 0.5, 1.0, 1.5, 2.0 mg/l) plant growth regulators. After four-six weeks of culture period, the cultures were transferred to the sub-culture medium having the same content as the induction medium. Then the cultures were transferred to the regeneration media containing different concentrations of 2,4-D or NAA (0, 0.1, 0.25 mg/l) in combination with BAP or Kinetin (0.25, 0.5, 1.0 mg/l). ？MS medium which does not contain growth regulators was used to root the shoots. As a result of the research, it was determined that the apical or axillary bud explants were the most suitable explant for multiple shoot formation and plantlet regeneration and that MS medium with 1.5 mg/l BAP alone was the most suitable medium. ？MS medium was succesful for rooting the shoots growing on the medium with BAP. However single shoots with roots were obtained in the induction medium supplemented with 1.0 mg/l NAA. The highest shoot regeneration ratio (an average of 36.0 shoots per explants) and roooting ratio (81.2%) was obtained from the axillary bud explants cultured in the medium supplemented with 1.5 mg/l BAP