Evaluation of Human Papilloma Virus (HPV) Existance with Molecular Methods in Sexually Active Women

The aim of this study was to determine HPV DNA by PCR in smear and biopsy materials in patients with abnormal smear and to compare the results with biopsy. The study included 24 patients aged 26 to 74 years. Patients with H-SIL, L-SIL or ASCUS who were diagnosed with abnormal smear during the pathological evaluation were evaluated and the presence of HPV was investigated with molecular tests. Demographic information was obtained from patients before colposcopy and the presence of factors that may cause HPV infection were examined. In pathological specimens taken from patients with abnormal smear results, biopsy was diagnosed as cervical CA in 33%, H-SIL in 4%, L-SIL in 13%, and normal in 50% of patients. HPV DNA was determined, and it was observed that HPV 16 was the highest prevalence of HPV subtypes (HPV 16, HPV 18, HPV 31 and HPV 58). Of the 5 patients with HPV 16, 4 were found to have cervical CA, while the remaining 1 was H-SIL. In addition, the HPV positivity rate was higher in people with more than 1 partner, whereas the number of HPV positive cases was three or more in gravity. In this study; the contribution of pathological diagnostic methods and microbiological results to the diagnosis, and the importance of the scanning programs and early diagnosis was revealed