Comparison of DNA Isolation Methods From Insect Tissues in Terms of Quality, Yield and Cost

Today, there are many studies conducted in the areas such as metagenomics, biotechnology, whole genome sequencing, genome evolution, and so on parallel to the developments in molecular biology and bioinformatics in molecular biology and bioinformatics. The first and most important step in studies with insect species is the use of an effective DNA isolation method. Small body and external chitin skeleton of the insects cause difficulties in isolating the insect DNAs. In this study, nine different DNA isolation methods were applied to carry out total DNA isolation from the individuals belonging to the species Cephus pygmeus (Linnaeus, 1767). The results were interpreted in terms of DNA yield, purity, cost, time and PCR performance criteria. When the cost and the toxicity of the used chemicals are taken into consideration, it is interpreted that the salting out method is the most suitable method for DNA isolation from insect tissues