Quantitative Analysis of Ciprofloxacin in an Ophthalmic Solution Using UV Absorption Spectrophotometry and Derivative Spectrophotometry

The quantitative analysis of ciprofloxacin in an ophthalmic solution was performed by UV absorption spectrophotometry and first derivative spectrophotometry. The UV absorption spectra of standard series and samples containing ciprofloxacin were recorded. Then, the derivative of UV absorption spectra was obtained for the derivative spectrophotometric analysis of the related drug. Linear regression equations in the linear concentration range of 3.0-28.0 μg/mL for analysis of ciprofloxacin were obtained by using direct absorbance measurement at 278.9 nm and using the dA/dλ values at 283.2 nm. The recovery assay results were found between 97.8 to 102.0 %. It was observed that the limits of detection and quantification were 0.74 and 2.74 μg/mL for UV absorption spectrophotometry and 0.60 and 2.00 μg/mL for first derivative spectrophotometry, respectively. Analysis results indicated that the proposed methods were precise, accurate and reliable for the determination of ciprofloxacin in commercial samples