The Effects of Achillea millefolium (Yarrow) Extract and Luteolin on In Vitro Rat Urinary Bladder Muscles

Yarrow (CP) is commonly used in alternative medicine and in its structure is found Luteolin together with many compounds. In this study, effects of CP methanol extract and Luteolin on isolated rat bladder smooth muscle contractility were investigated. In the study, twenty male Sprague Dawley rats were used and the rats were decapitated under anesthesia. The cleaning of isolated bladders were done and muscle strips prepared from the bladders were placed to organ baths. The tissues were applied to 1 gram tesnion and left for 1 hour incubation period. At the end of the incubation period, CP extract, Luteolin, acetylcholine (ACh), potassium chloride (KCl) and Nifedipine was applied with a protocol the bath. Data were evaluated by Tukey test in one way ANOVA. It was determined that different doses of CP extract and Luteolin inhibited the isolated bladder contractility induced by ACh and KCl. It was seen that of the contractions induced by ACh submaximal dose more strongly suppressed by CP extract and Luteolin in the presence of Nifedipine in the bath. Subcutaneous dose-induced indications of KCl were found to be more inhibitory in co-existence of CP extract and Nifedipine. It was determined that together with CP extract and Nifedipine were more inhibited the KCl’s submaximal dose-induced contractions, but but the effect of Luteolin in the presence of Nifedipine in bath was not changed. In this study, it was assigned that inhibiting the bladder smooth muscle contractions of different doses of CP extract and Luteolin and of CP extract to be also effective on the M3 receptor pathway