Effect of 7-Dehydrocholesterol on Ram Semen Spermatological Parameters on Short Term Preservation

The aim of this study was investigated to effect of 7-dehydrocholesterol loaded cyclodextrin (7-DCLC) on ram semen spermatological parameters on short term preservation at +4 ？C. Collected semen with electroejaculator at non-breeding season. Each animal native ejaculates were mixed and divided into 4 equal groups, diluated with tris/egg yolk extender with included different rates of 7-DCLC (1.5 mg, 3.0 mg and 5 mg/120x106) and control (C). After extended semen samples were preservation at +4 ？C and evaluated motility, membrane integrity and abnormal spermatozoa rate until 96. hour. There were no significant differences between the groups on abnormal spermatozoa rate (P>0.05); there were a significant differences between the groups on motility at all storage period (P<0.05).The highest motility were observed in 7-DCLC 1.5 mg at 72. and 96. hour (P<0.05). The highest dose (5 mg/120x106) 7-DCLC were decreased the motility and membrane integrity compared to other groups. Besides, 7-DCLC 3.0 mg group membrane integrity was lower than control at 72. hour (P<0.05). In conclusion, tris/egg yolk extender which is contain 7-DCLC 1.5 mg were protected to ram semen motility and membrane integrity up to 96. hour at +4°C preservation