The Relationship of Myosin Heavy Chain 8 (MyH8) Gene Expression Profile of Live Weight in Slaughtered the Simmental and Brown Swiss Cattle Breeds which Reared in Turkey

The aim of this study was to investigate, the relationship between of MyH8 gene expression profile of the liver and muscle tissue with the live weight, in the Simmental and Brown Swiss cattle. Simental (n = 40) and Brown Swiss (n = 40) male cattle were used in this study. Before slaughter, live weight measurements, muscle and liver tissue were taken and tissues were frozen in liquid nitrogen. It was made respectively RNA isolation by Trizol from tissue, c-DNA synthesis from RNAs and expression analysis by SYBR Green method. There was no significant correlation between live weights and MyH8 gene expression in muscle and liver (P>0.05). There was no significant difference in muscle MyH8 gene expression between Simmental and Brown Swiss (P>0.05). However, the difference in MyH8 gene expression in the liver was significant for two breeds (P<0.001). MyH8 gene expression level in Brown Swiss liver was significantly higher than the Simmental. As a result, the data obtained from study indicate that there is no significant relationship between the expression level of the MyH8 gene and live weight in the Simmental and Brown Swiss, but the MyH8 gene has the potential to carry a different expression profile in these breeds