Determination of apple proliferation phytoplasma disease (Candidatus Phytoplasma mali) in apple orchards in Adana and ？？el provinces

Aims: In this study, the presence of Apple Proliferation (Candidatus Pyhtoplasma mali) was investigated in Adana and ？？el provinces. Methods and Results: Surveys were conducted in apple growing areas of Adana and ？？el provinces between 2013-2016 and 234 trees in 39 different orchards were investigated for phytoplasma diseases. DNA isolations of samples, PCR and RFLP (Restriction Fragment Length Polymorphism) analysis were performed. Amplicated fragments of 14 out of 234 samples tested by PCR/RFLP analysis observed as same as “Candidatus Pyhtoplasma mali” positive control. Conclusions: Apple (Malus domestica Borkh.) is among the temperate pome fruits that has been important production areas, and grown for many years in our country. AP is listed in quarantine in our country and economically limits apple production in the world. So that, the condition of the disease is important in the apple growing areas. Significance and Impact of the Study: This study was conducted to determine the presence of AP in Adana and ？？el provinces, and especially was to reveal the presence of phytoplasma infection in ？？el