Possibility of the Use of Embryo Culture Technique to Improve Resistant Lines Against Cabbage Clubroot (Plasmodiophora brassicae Woronin) via Interspesific Hybridization

ABSTRCT: This research was carried out at Ondokuz May？s University between 2010 and 2014. Breeding of resistant lines against to clubroot (Plasmodiophora brassicae) via interspesific hybridisation between B. oleraceae L. and B. rapa L. In these research; 831 flowers were hybridisated between B. oreacea L. var. Dürme and B. rapa L. Lines ECD-1, ECD-2, ECD-3 and ECD-4. 2663 primitif embrios were transfered to medium and produced 813 haploid and 348 double haploid plants. 162 double haploid plants trasfered green condition after vernalisation and self crossing was done in flowering condition. 293 seeds were harvested from 8 hybrid plants from DurmexECD-4 hybrid combination. In order to test the endurance status of cabbage clubroot disease, 95 hybrid double-haploid plants were cultivated by seeding the obtained seeds. After the test, a gene pool was formed, consisting of 33 plants that tolerate cabbage root diseas