Antioxidant properties of Melissa officinalis L. callus cultures

Melissa officinalis L. is a Mediterranean plant from the family of the Labitae, and has lemon fragrance. Melissa is a perennial herbaceous plant that forms the plant size between 60-100 cm. It is a known and used plant for a long time, because of its pleasant smell. Because it is effective in beekeeping, this plant is called as the swarm plant. It is a medicinal and aromatic plant, since it contains essential oils containing citral, stranellal, linalool and pinemia and flavonoid and resin. The antioxidant activity of the in vitro produced calli was determined in this study, which was also determined by previous studies. Melissa officinalis L. nodals were used as an explant source. Nodules were promoted with different plant growth regulator combinations for callus formation in Murashige Skoog medium. The antioxidant activity of the resulting callus was determined by the ABTS method and the results were given as "TEAC (tropoxyl equivalent antioxidant capacity) equivalent". The highest antioxidant capacity (0.365 mmol / L) was seen in 1.5 mg / L 2,4-D + 1 mg / L PIC + 0.5 mg / L KIN-induced callus. The lowest value (0.191 mmol / L) was obtained from calli promoted with 1.5 mg / L 2,4-D + 0.5 mg / L BAP