Determination of antioxidant and antiradical properties of Picea orientalis cone

Structural degradation of DNA, proteins, carbohydrates and lipids occurs as long as the reactive oxygen species formed in metabolism are not inhibited. As a result, both the stabilization and the function of the cell membrane are impaired and many degenerative diseases occur. In previous times, people applied to herbal remedies to solve their health problems. Today, scientific studies have increased, especially as alternative medicine has shown. Plants containing phenolic compounds are commonly used as natural antioxidants. These compounds stop or prevent the reactions caused by free radicals and prevent diseases such as heart, cancer, and lung diseases. Antioxidants are known as substances that prevent or completely stop the oxidation of biomolecules and they have use a very common. East spruce is the scientific name P. orientalis and it found in the family of pine trees. In this study, it was aimed to determine the antioxidant capacity by using ethanol extracts of east spruce cone. For this purpose, antioxidant and antiradical properties were determined by different bioanalytical methods in vitro such as determination of iron and copper reduction capacities, ABTS and DPPH radical scavenging activities, total phenolic and flavonoid compound amounts by using ethanol extracts of east spruce cone. The P. orientalis cone had an IC50 value of 24.75 μg mL-1 for DPPH radical removal activity and 13.42 μg mL-1 for ABTS radical removal activity. Accordingly, it has been determined that the methods have high antioxidant capacity