Developing Lateral Flow Assay Based Rapid Diagnosis Kit for Serologic Diagnosis of Cattle Brucellosis

Brusellosis caused by microorganisms of Brucellagenus is a worldwide zoonotic infection seen in livestock and has serious risk for human and animal health and negative effect on animal production. The disease agents infect especially genital organs like uterus, testes, mammary glands and cause abortion and infertility in livestock. The aim of this study was to develop a rapid lateral flow test (LTF) for diagnosis cattle brucellosis. Sensitivity, specificity and diagnostic accuracy of LFT were evaluated by comparing the results with Rose Bengal Plate Test (RBPT), complement fixation test (CFT) and i-ELISA. Out of 91 serum samples 45 (49.5%), 44 (48.3%), 37 (40.7%) and 34 (37.4%) were found positive by RBPT, i-ELISA, CFT and LFT, respectively. Based on the results by CFT as reference test, sensitivity and specificity of LFT were found 92% and 100%, respectively. As conclusion, LFT and RBPT gave similar results and it was thought that LFT is an easy, rapid and specific screening test for diagnosis cattle brucellosis in the field