Toxoplasma gondii Analysis in Water and A New Approach: Loopmediated ？sothermal Amplification Technique

Toxoplasma gondii is an obligate intracellular protozoan parasite. It is one of the most common disease seen worldwide and can affect all the vertebrates. Toxoplasmosis is a disease caused by T. gondii and least one-third of the world’s population has detectable T. gondii antibodies. Seroprevalence of the disease is approximately 40% in Turkish population. Humans become infected with T. gondii mainly by ingesting uncooked meat containing viable tissue cysts or by ingesting food or water contaminated with oocysts from the feces of infected cats. Circumstantial evidence suggests that oocyst-induced infections in humans are clinically more severe than tissue cyst-acquired infections. Until recently, waterborne transmission of T. gondii was considered uncommon. But the epidemics of toxoplasmosis made the topic became an agenda of the World again. That's why it is a must to take the necessary precautions and analyses should be done for the disease which is epidemic throughout water. Nowadays water analysis for the agent is mostly conducted with molecular studies, especially Polymerase Chain Reaction (PCR) and Loopmediated isothermal amplification (LAMP). This review puts emphasis on the importance of disregcharded Toxoplasma which can be plagued throughout water and also will highlight the necessary precautions that should be taken