Early Treatment with Metformin Decreases Pancreatic Damage in Rats with LPS Induced Sepsis

In this study, the effects of metformin on pancreatic tissue after lipopolysaccharide (LPS)-induced sepsis were investigated. 30 Sprague Dawley male rats were used in the study. Five groups were formed: control, sepsis, sepsis+1 hour before metformin, sepsis+1 hour later metformin and sepsis+3 hour later metformin as 6 animals in each group. LPS and metformin was prepared at 5 mg/kg and 200 mg/kg volumes, respectively, and injected intraperitoneally to the rats. Blood samples and pancreas tissues were taken from the rats 24 hours after LPS injection. Amylase, glucose and insulin parameters were measured in serum of rats. Malondialdehyde (MDA) and myeloperoxidase (MPO) parameters in pancreas tissues of rats were evaluated. Pancreatic tissues were examined by hematoxylin-eosin (H-E) staining method histopathologically. When the results were evaluated, it was seen that LPS caused sepsis and pancreatic tissue damage in rats. However, it has been determined that metformin significantly alleviates these damages in the treatment groups. In particular, metformin administered prior to sepsis has been shown to have protective effects in the pancreatic tissues of rats