DNA METHYLATION PROFILES OF GENES EFFECTIVE IN PLACENTAL ANGIOGENESIS FOR PREGNANTS WITH GESTATIONAL DIABETES

The reason of absence preventive processes from GDM and early treatment modalities is unsettled ethiopathologies. Defining the role of the placenta is important for these pathologies. Fetal growth is adjusted due to placental genetic and epigenetic factors. PURPOSE: DNA methylation which is an epigenetic mechanism, is modifiable and utiziable for diagnosis and treatment recently. DNA methylation changes of VEGF, sFLT-1, PIGF genes, have roles on placentation were evaluated according to GDM MATERIAL and METHOD: All placental samples had taken from pregnants who routinely followed at Suleyman Demirel University Gynecology and Obstetrics Department, compatible with study criteria and diagnosed as GDM (n=15) and healthy pregnants (n=17). DNA Methylation levels were analysed by ‘New Generation DNA Sequencing’ method. Data collections analysed via SPSS 23 programme. Because of data distrubution Manny Whitney U tests are used for means; SPEARMAN correlation analysis is used to reveal relation between datas. RESULT: The methylation levels were not changed significantly due to demographic characteristics. The analyses that examine differences in methylation levels of PIGF were established no statistically significant difference. There was statistically significant difference in levels of methylation of sFLT-1 gene at the position of P92186. , P92344. , P92456. primer regions as hypomethylated and VEGF gene at the position of P92668. , P92710. , P92863. primer regions as hypermethylated. CONCLUSION: The findings of our study indicates changes of DNA methylation in some regions of VEGF, sFLT-1 genes and it is compatible with literature. But clarifying of related regions of genes via whole genom studies with a large population is necessary to reach predictive values