High Resolution Automatic Panoramic Imaging by Maintaining Optimal Range for Cytopathological Analysis

Since the microscope has a small field of view, pathologists can only see a certain part of the specimen during the cytopathological analysis process. In order to see the whole area of the sample, they scan the sample in three dimensions by moving the microscope platform in the X-Y-Z direction. The aim of the study is to obtain a high resolution panoramic image of the sample by automating the process of cytopathological analysis. Literature studies for the automation of the panoramic imaging process do not take into account the depth of focus measured in microns, which is present in the microscope, while creating images with the same field of view. This results in differences in focus between the images and during the scanning process blurred images are obtained. In order to solve this problem, the depth of focus is extended to produce optimum focused images. To evaluate the success of the proposed method, panoramic images were obtained using two different scanning processes suggested in the literature. The generated panoramic images are compared using the metrics without requiring reference image and the success of the proposed method is proved by both quantitative and visual result