Introduction to Chromatography, Basic Working Tips for High Performance Liquid Chromatography

Chromatography that finds usage in a wide range of fields from medical laboratories to the pharmaceutical industry, from food to veterinary medicine, agriculture, chemistry to different industrial branches; is used for various purposes such as measurement, separation and purification. The most important, effective and common use of chromatography by various methods for different fields is liquid chromatography which is based on high performance liquid chromatography (HPLC) and LC-MS/MS devices. Chromatography is a method used to separate and purify the compounds in a mixture. The main aim of chromatography is separation and measurement of the amount of our target sample. This method is based on the principle of moving or drifting the various materials at different speeds on a stationary phase by a mobile phase. The purpose of this article is to introduce an overview of chromatographic methods, followed by introduction of by descending to the basics of HPLC and to give brief information about a sample analysis and method development step. We hope that this article will shed light for researchers and technicians in developing methods and working at HPLC and LC-MS / MS instruments