Investigation of Hyaluronidase Activity with Amniotic Fluid on Romanov Sheep

Bovine testicular hyaluronidase (BTH) is a very important enzyme in industrial field, cancer studies and in many animal applications. In this study, it was investigated whether amniotic fluid of Romanov sheep could be used as an alternative new substrate source for BTH enzyme. As a result of the study, the BTH activity was found as 9.187 U mL-1 with commercially available hyaluronic acid (HA), while the BTH activity of 35 different amniotic fluids was found between 1.604-18.659 U mL-1 . No information was reported on whether the amniotic fluids of the Romanov breed sheep were a source of substrate for the BTH enzyme due to the HA content. It was thought that amniotic fluid of the Romanov breed sheep could be a new HA source, as the BTH activity calculated using the commercial HA and the BTH activities obtained using amniotic fluid were very close