Design of Reversed-Phase Chromatography Column Packing Material for Protein Separation: Part 2- Chromatographic Performance

In the second part of the two-part study, monodisperse and porous poly(vinyl acetate-co-divinylbenzene) [poly(VAc-co-DVB)] and poly(divinylbenzene) [poly(DVB)] column packing materials synthesized in the first part of the study were tested in Reverse Phase Chromatography (RPC) under different conditions. The investigation of column performances concluded that columns packed with poly(VAc-co-DVB) (2500 plate/m) and poly(DVB) (1500 plate/m), showed high and stable theoretical plate number and high peak resolution values compared to literature values. Protein recovery data in RPC column, poly(VAc-co-DVB) (93-99%), were determined as similar as quantitative values for all proteins. Reproducibility tests of analytes of poly(VAc-co-DVB) column were assessed "run to run" and "day to day" Relative Standard Deviation (RSD) values were determined under 2.8%