Comparison Of Some Effects Of Wild-Grown And Cultivated Forms Of Ganoderma Lucidum In Turkey On Chicken Embryos

Ganoderma lucidum (Curtis) P. Karst is a mushroom which used for alternative medicine worldwide particularly in China, also known as “Immortality Mushroom or Reishi”. In recent years, cultivated G. lucidum is also produced in Turkey and consumed by people especially as tea, coffee and tablets. The aim of this study was to determine the some effects of wild-grown and cultivated G. lucidum in Turkey on the development of chicken embryos, as an alternative experimental animal, for the first time. Two experiments were carried out for this purpose. Wild-grown and cultivated forms of G. lucidum were tested at the same doses in the first and second experiment, respectively. Aqueous extracts of G. lucidum at different doses (1750 μg/egg, 875 μg/egg and 219 μg/egg) were injected into the fertilized chicken eggs at 8th day of incubation as well as sterile bi-distilled water for control groups. Following parameters were examined on 11th day of the incubation: rates of dead and abnormal embryo, malformation types, live and relative embryo weights. In addition, some of the embryos were totally stained with Alizarin Red-S method for bone development. Both wild-grown and cultivated forms of G. lucidum at doses tested did not present significant embryotoxic and teratogenic effects as well as did not affect the bone development of embryos at the macroscopic level