ANTIMICROBIAL ACTIVITY OF CLEMENTINE PEEL ESSENTIAL OIL WITH ITS CYTOTOXIC AND IN VITRO WOUND HEALING POTENTIAL ON NIH-3T3 FIBROBLAST CELLS

Citrus essential oils has many area of use such as skin and hair care, insomnia, improving digestion, and boosting immune system. The present study is aimed to determine the biological activities of clementine (Citrus clementina Hort. ex Tan), one of the mandarin species, peel essential oil (CPEO) that grow in Turkey. Antimicrobial activity of CPEO was evaluated using disc diffusion method against Escherichia coli, Staphylococcus aureus, and a fungi Candida albicans. The MTT (3-[4, 5-dimethylthiazol-2-yl]-2, 5 diphenyltetrazolium bromide)-colorimetric monocyte mediated cytotoxicity assay was applied using NIH-3T3 cells and IC50 value was calculated. CPEO was also evaluated for its in vitro wound healing effect on the fibroblast cell migration and proliferation using scratch wound assay technique. Results of the present study indicated that CPEO has remarkable antimicrobial activity. The highest inhibition zone was observed against C. albicans as 20.67±0.58 mm. IC50 value obtained for CPEO was 52.50 μg/mL. Fibroblast cells showed higher migration than the control group to heal the artificial scar after treatment of CPEO concentrations below the IC50 dose. The results of the present study indicated that the CPEO may be useful in effective management of skin care applications and wound healing products with its potent activity against pathogen microorganisms