HPLC Analysis of Phenolic Compounds from Gypsophila aucheri Boiss. and Investigation of Antioxidant and Cytotoxic Activity of Gypsophila aucheri Boiss. exracts

This study was designed to investigate the antioxidant and antiproliferative activities of Gypsophila aucheri Boiss. extracts as well as phenolic content by RP-HPLC technique. Antioxidant activity potentials of the extracts were evaluated by four different methods namely, 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging capacity tests, cupric ion reducing antioxidant capacity (CUPRAC) method, and metal chelating assay. Antiproliferative activities of the extracts were tested against breast carcinoma (MCF-7), colorectal carcinoma (HT-29), and hepatocellular carcinoma (HepG2) cells. RP-HPLC analysis revealed the presence of eight phenolic compounds in eighteen phenolics scanned. Antioxidant activity results showed that methanol extract of Gypsophila aucheri displayed more pronounced antioxidant activity than water extract according to DPPH and ABTS tests, whereas water extract was superior than methanol extract for CUPRAC and metal chelating activity tests. Cytotoxicity tests demostrated the promising antiproliferative activity of methanol extract against all the tested cancer cell lines with its lower IC50 values. However, both extracts were observed to display moderate biological activity compared to positive controls. The obtained data suggest that Gypsophila aucheri could be evaluated as a promising source for food and nutraceutical industries due to its striking antioxidant and moderate antiproliferative potentials together with high phytochemical profil