Detection of Barley yellow dwarf virus-PAV and Barley yellow dwarf virus-MAV in wheat production areas of Isparta and Afyonkarahisar provinces

Barley yellow dwarf disease is the most widespread viral disease causing economic losses in wheat areas all around the world. Any species of Barley yellow dwarf viruses (BYDVs) of the Luteoviridae family causes the disease. In this study, Barley yellow dwarf virus PAV (BYDV-PAV) and Barley yellow dwarf virus MAV (BYDV-MAV) were identified in wheat growing areas of Afyonkarahisar and Isparta provinces, a total of 94 samples were collected from wheat plants exhibiting virus-like symptoms in 2016 growing season. BYDV-PAV and BYDV-MAV were determined by using DAS-ELISA (Double antibody sandwich enzyme linked immunosorbent assay). The result of DAS-ELISA revealed that 68 out of 94 samples at the rate of 72.3 % were found to be infected with BYDV-PAV and two of them at the rate of 2.1 % had BYDV-MAV. The mixed infection of both viruses with a rate of 4.2 % was detected in only four of the samples tested