Determination of Extracellular Hydrolytic Enzyme Production Capabilities of Acidithiobacillus Bacteria Isolated from Awasppi River in Sulaymaniyah, Iraq and 16S rDNA Analysis

In this study, the 16S rDNA gene regions’ phylogenetic positions based on the sequence analysis were determined by examining some hydrolytic extracellular enzyme activities of the Acidithiobacillus bacteria isolated from the water and mud samples obtained from the Awasspi River in Sulaymaniyah province, northern Iraq and from the Darzilais sulphur cave in Sangaw district. While the catalase activity of the isolates was positive; lipase, protease, xylanase, and amylase activities were found to be negative. Genomic DNAs of the isolates were isolated and 16S rDNA gene region was realized with PCR amplification. To determine the distance between the species, Maximum Likelihood algorithm was used to form a phylogenetic tree. It was observed that gene strands belonging to Acidithiobacillus species acquired from NCBI and 2 isolates we used in our study clustered with a strong homology and their genetic positions were revealed