The Effects of Adding Different Levels of Lactic Acid Bacterial with Enzymes Inoculants on the Fermentation, Aerobic Stability and in vitro Digestibility of Wet Brewers Grain Silages

This study was carried out to determine the effect of lactic acid bacteria+enzyme (LAB+E) mixture inoculants on the fermentation, aerobic stability, and in vitro organic matter digestibility (IVOMD) of wet brewers grain silages. SILAID (Global Nutritech Biotechnology LLC, Richmond, VA) containing water soluble Lactobacillus plantarum and Enterococcus faecium bacteria with cellulose, pentosanaz and amylase was used as bacterial inoculants. Inoculants were applied to silages at 5x105, 1x106 and 5x106 cfu/g levels. Wet brewers grains were ensiled in 1.0 litter special jars equipped with a lid that enabled gas release only. Silages were sampled for chemical and microbiological analyses on day 60 after ensiling and subjected to aerobic stability test for 5 days. In addition, IVOMD of silage was determined with enzymatic technique. Lactic acid bacteria+enzyme mixture inoculants decreased the pH, ammonia nitrogen and acetic acid whereas increased the lactic acid and lactobacilli count of silages (P<0.05). In addition, LAB+E mixture inoculants decreased neutral detergent fiber, acid detergent fiber and cellulose contents (P<0.05) whereas were not effect IVOMD of silages (P>0.05)