Analysis of Somaclonal Variation in Natural Tetraploid Trifolium pratense L. Regenerants Using RAPD Markers

Trifolium pratense L. is an agricultural legume which is grown mainly in Europe, North America, and Anatolia. As the plant shows great diversity in Anatolia, Anatolia was accepted as the center of the origin of T. pratense L. This study aimed to analyze somaclonal variation among natural tetraploid T. pratense L. regenerants propagated in vitro and their donor plants by using RAPD techniques. In this study, for regeneration, the apical meristems of aseptic plants germinated on hormone-free MS medium were used, then they were transfered on PC - L2 medium containing 1 mg/l NAA + 3 mg/l BAP. In this research, RAPD techniques including 8 different primers were used to detect somaclonal variation. As a result, all of the primers showed polymorphism. The lowest polymorphism percentage was seen between number 2 clone and donor with 17.07% (D2-C2), the highest polymorphism percentage was seen between number 4 clone and donor with 60.21% (D4-C4). The highest variation difference was seen between donors D2 (a total of 123 bands) and D6 (total 86 bands)