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OALib Journal期刊
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-  2019 

A Scalable, Multiplexed Assay for Decoding GPCR-Ligand Interactions with RNA Sequencing

DOI: https://doi.org/10.1016/j.cels.2019.02.009

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Abstract:

? RNA-seq-based approach for discovering olfactory receptor-odorant pairs ? Extensive cell line engineering allows stable expression of difficult receptors ? 79 novel interactions were discovered, and 15 olfactory receptors were de-orphanized ? Platform can be extended to other GPCRs or drug target

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