Improving Estimates of Compensatory cis–trans Regulatory Divergence

In this article, I regretfully neglected to cite a paper [ 1 Emerson J.J. et al. Natural selection on cis and trans regulation in yeasts. Genome Res. 2010; 20: 826-836 Crossref PubMed Scopus (0) Google Scholar ] in which the authors proposed separating RNA-seq technical replicates (the same library sequenced on different lanes) to estimate cis and trans components of gene expression divergence. While similar in spirit to the approach that I proposed, it should be noted that separating technical but not biological replicates will still lead to an artifactual negative cis–trans correlation if there is any error in allele-specific expression estimates driven either by biological variation among samples or by variation in library construction (e.g., due to PCR ‘jackpots’)