Signal Analysis of Nanopore RNA Sequencing to Interrogate Poly(A) Tails and Post-Transcriptional Modifications

Although development of high throughput RNA sequencing technologies has allowed significant advances in our understanding of transcriptome complexity and regulation, certain questions remain intractable with conventional short read cDNA sequencing. Native RNA sequencing technology can address these difficult questions; including identity and frequency of splice variants, full-length poly(A) tail assessment, base modifications and transcript haplotyping. To accomplish this, we leveraged our extensive consortia-generated GM12878 native RNA dataset to showcase this exciting technology and the promise it holds for the direct and simultaneous interrogation of these RNA features