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-  2019 

Draft genome of the big-headed turtle Platysternon megacephalum

DOI: https://doi.org/10.1038/s41597-019-0067-9

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Abstract:

The big-headed turtle, Platysternon megacephalum, as the sole member of the monotypic family Platysternidae, has a number of distinct characteristics including an extra-large head, long tail, flat carapace, and a preference for low water temperature environments. We performed whole genome sequencing, assembly, and gene annotation of an adult male big-headed turtle based on the Illumina HiSeq X genomic sequencing platform. We generated ~497.1?Gb of raw sequencing data (×208.9 depth) and produced a draft genome with a total length of 2.32?Gb and contig and scaffold N50 sizes of 41.8?kb and 7.22?Mb, respectively. We also identified 924?Mb (39.84%) of repetitive sequences, 25,995 protein-coding genes, and 19,177 non-coding RNAs. We generated the first de novo genome of the big-headed turtle; these data will be essential to the further understanding and exploration of the genomic innovations and molecular mechanisms contributing to its unique morphology and physiological features

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